Answer: No, under natural conditions a queen will often mate with several different toms during one period of oestrus (over three to four days), sometimes with more then one in a day. This makes it possible for at least two different tom cats to fertilize different ages, so that kittens in the same litter can have different fathers, a phenomenon knows as super fecundation. Consequently, if you wish to obtain kitten from the breeding of two particular cats it is important not to allow the queen access to other males, even after the planned mating has taken place.
The male cat needs to be familiar with the area in which mating is to take place, and increases his feeding of security by spraying and rubbing against the surroundings. For this reason matings arranged by cat breeders generally involve taking the queen to the premises of the stud tom and not the other way around.